Establishment of cloned homogeneous immunoassay for detection of P53 protein in serum and stool samples from patients with gastric carcinoma
- VernacularTitle:克隆酶均相免疫技术测定胃癌患者血清和粪便P53蛋白的研究
- Author:
Jiangen SHEN
;
Jie LIN
;
Yongliang ZHU
;
Jun ZHANG
;
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Protein P53;
Homogeneous immunoassay
- From:
Chinese Journal of Laboratory Medicine
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate reliability of the cloned homogeneous immunoassay technique for detection of P53 protein content in serum and stool from patients with gastric carcinoma. Methods To prepare EA, ED and ED P53 fusion protein of beta galactosidase by PGEX 4T 2 expression vector, a novel cloned homegeneous immunoassay was establishment for detection of P53 protein in serum and stool samples from patients with gastric carcinoma and non gastric carcinoma groups as well as healthy control. Results The concentration of P53 protein in 31 gastric carcinoma subjects were remarkably increased as compared with 56 non gastric carcinoma ( P
