Biological Features of Mouse Macrophage Transfected with Toxoplasma gondii GRA-1 Gene
- VernacularTitle:转染弓形虫致密颗粒蛋白-1编码基因的小鼠巨噬细胞生物学特性
- Author:
Biwen PENG
;
Jianyin LIN
;
Tao ZHANG
;
Mingsen JIANG
;
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
Macrophage;
Toxoplasma gondii GRA-1 genes;
Transfection;
Biological feature
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of biological features of macrophages after transfected by Toxoplasma gondii GRA 1 genes (P24). Methods The transfected cells Cyto P24 RAW264.7, Nuc P24 RAW264.7, Mito P24 RAW264.7 and ER P24 RAW264.7 were studied by RT PCR to determine the P24 mRNA expression. Growth features of the cells were examined with microscopy and the cell growth curve was developed. Results In four cell lines, expression of ER P24 RAW 264.7 was found to be higher than the other three, and there was no P24 mRNA expression in either of the cells without P24 insert. The attachment and the proliferation of ER-P24-RAW264.7 were more rapid than normal RAW264.7. Conclusion Transfection of mouse macrophages ER RAW264.7 strain with T. gondii P24 gene leads to a prominent change of biological features in the studied cell line.
