Preparation and testing of hepatitis C core antigen ELISA reagent
- VernacularTitle:丙型肝炎病毒核心抗原检测试剂的研制及初步应用
- Author:
Guohua WANG
;
Heqiu ZHANG
;
Shaobo LI
;
Shaogang LIU
;
Kun CHEN
;
Xiaoguo SONG
;
Shigan LING
;
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus;
Hepatitis C core antigen;
Monoclonal antibody
- From:
Chinese Journal of Blood Transfusion
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a sandwich enzyme-linked immunosorbent (ELISA) method for early diagnosis of hepatitis C. Methods Immunization of Balb/c mice with hepatitis C core antigen were prepared by genetic engineering. Mouse monoclonal antibodies (McAb) to anti-HCV-core Ag were obtained. A sandwich ELISA kit for detecting HCV-core Ag was developed by using four strains of anti-HCV-core Ag McAb. One hundred and twenty five serum specimens with increased ALT but negative for anti-HCV, anti-HIV, and anti-Tp tests were tested. Results Nine of the 125 specimens were positive for HCV-core Ag. Conclusion The double sandwich ELISA kits for detecting HCV-core Ag may be useful for the early diagnosis of hepatitis C .
