Isolation and Characterization of Collagenolytic Enzyme-Producing Strain from Rotten Hides and Primary Analysis of the Enzyme Property
- VernacularTitle:具胶原蛋白酶活性铜绿假单胞菌的筛选
- Author:
Guang-Yao YANG
;
Jun XIE
;
Ning XU
;
Ling LI
;
Yi-Zheng ZHANG
;
- Publication Type:Journal Article
- Keywords:
Collagenase, P.aeraginosa.
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Bacteria isolated from rotten hides,one identified as Pseudomonas aeraginosa later, were shown to decompose fresh hides completely in 48 hours at room temperature. Strongly collagenolytic activity was detected when native collagen from calfskin was used as substrate. The optimal reaction pH value and temperature of the collagenace produced by P.aeraginosa are 7.5 and 32℃ respectively. EDTA and EGTA inhabit the collagenolytic activity severely while PMSF has little effect on it. Not only media components but also fermentation conditions have differents effect on the production of this collagenolyic enzyme.
