Studies on the Bacterial Diversities of the Hot Springs at Tengchong Rehai in Yunnan Province China
- VernacularTitle:云南腾冲热海三热泉细菌多样性的研究
- Author:
Qin-Yuan LI
;
Xiao-Long CUI
;
Dong-Hua ZHANG
;
Qian PENG
;
Li-Hua XU
;
- Publication Type:Journal Article
- Keywords:
DGGE, Hot spring, Bacterial biodiversity
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Bacterial biodiversities of microbial mat and sediments, which were sampled from thermal springs of Tengchong Rehai in Yunnan, were preliminarily studied with PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Directly extracted total DNA from environmental samples amplified by PCR with two sets of bacteria-specific primers. The PCR products, which include the V 8 and V 9 high-variable regions respectively, were analyzed by using DGGE. The DGGE profiles not only indicated the existence of higher levels of bacterial diversity, but also showed that the microbial mat and sediments have different dominant bacteria. Furthermore, the bacterial PCR-DGGE displayed clear profiles of bacterial structure selected by the key abiotic factors of the extreme environments, such as temperature and concentration of oxygen.
