Immobilization of Fungal Laccase on Nylon Net and Application of the Immobilized Enzyme
- VernacularTitle:白腐真菌漆酶的固定化及其应用研究
- Author:
Shu-Xiang ZHANG
;
Ya-Zhong XIAO
;
Yi-Ping WANG
;
Min ZHANG
;
- Publication Type:Journal Article
- Keywords:
Nylon net , Immobilization, Fungal laccase
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Condition optimization for immobilization of Fungal laccase with Nylon Net and glutaraldehyde and the nature of the immobilized enzyme were studied. The optimum conditions of the immobilization are: Nylon Net is crosslinked with 5% glutaraldehyde 15mL for 6 hours; the 30U laccase is added for immobilization for 8 hours. On this case, the recovery of enzyme activity was 50.3%. Compared with free enzyme, the thermal stability of immobilized enzyme was improved evidently but the optimal pH decreased slightly. 52% enzyme activity of immobilized laccase was hold after 8 cycles treatment with low concentration pulp wastewater.
