A Rapid Method for Preparation of Fungal Chromosome DNA
- VernacularTitle:一种快速提取真菌染色体DNA的方法
- Author:
Xiaoling ZHOU
;
Wei SHEN
;
Zhiming RAO
;
Zhengxiang WANG
;
Jian ZHUGE
;
- Publication Type:Journal Article
- Keywords:
Fungi, Chromosome DNA, Extract
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The article introduce a rapid method for preparation of fungal chromosome DNA In this method the quartz sand is used to break the fungal cell wall and the chromosome DNA is harvested rapidly in 1～2 h The method is applied successfully by the author to four kinds of fungi Neurospora crassa , Aspergillus oryzae , Morchella esculcnta , Saccharomyces cervisae All the chromosome DNA extracted has the fragment size lager than 20 kb and can be used directly for either digestion with restriction endoenzyme or PCR
