Effects of culture medium osmolarity on the proliferation of human hepatoma cells
- VernacularTitle:渗透浓度对肝癌细胞增殖的影响
- Author:
Jing TIAN
;
Yu WANG
;
Wenge TONG
;
Chenhao ZHANG
;
- Publication Type:Journal Article
- Keywords:
Osmolarity;
Hepatoma;
Proliferation
- From:
Journal of Medical Postgraduates
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of culture medium osmolarity on the proliferation of human hepatoma cell line HHCC. Methods:Cell proliferation was analyzed by MTT assay and cell cycle analysis was carried out by flow cytometry. Results:Hyperosmolarity decreased significantly the absorbance (A) of HHCC cells and cell number, and hyposmolarity have counter effects. Compared with control, hyperosmolarity blocked cell cycle progression at the G 1 phase, while hyposmolarity accelerated cells into S phase from G 1 phase, and the effect of hyposmolarity was weakened by chloride channel blocker NFA. Conclusion:Culture medium osmolarity influenced the proliferation of HHCC cells by mediating cell cycle.
