A Convenient Method to Select DNA Fragments of Cephalosporium acremonium with Promoter Function
- VernacularTitle:一个从顶头孢霉中筛选具有启动子功能的DNA片段的简便方法
- Author:
Pi-Yan ZHANG
;
Chun-Bao ZHU
;
Bao-Quan ZHU
;
Wen-Jie ZHAO
;
- Publication Type:Journal Article
- Keywords:
E. coli-yeast shuttle plasmid, Promoter-trap vector, Cephalosporium acremonium, Gene library, Fungi
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A promoter-trap vector pGBT14 for selecting promoters of fungus gene was constructed with E. coli-yeast shuttling plasmid pGBT9. Using this vector, a0. 5-2. 0kb chromosomal DNA library of Cepholosporium acremonium was constructed, and twenty four DNA fragments with promoter function in Saccharomyces oerevisiae Y153 were selected from this DNA library. And the promoter function of these DNA fragments was analyzed.
