ClONING OF NP, P AND L GENE OF NEWCASTLE DISEASE VIRUS OF GOOSE ORIGIN AND IDENTIFICATION OF P GENE EXPRESSION
- VernacularTitle:鹅源新城疫病毒NP、P和L基因的克隆与P基因的表达鉴定
- Author:
Yu-Liang LIU
;
Yan-Tao WU
;
Yong HUANG
;
Wei-Xing SHAO
;
Dong-Ping WEI
;
Xiu-Fan LIU
;
- Publication Type:Journal Article
- Keywords:
Goose, Newcastle disease virus, NP, P and L gene, Green fluorescence protein, Expression
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
NP, P and L gene of Newcastle disease virus of goose origin were amplified and cloned into pGEM-T easy vector and then subcloned into pCI-neo expression vector respectively, the positive clones were identified by enzyme cutting, PCR and sequencing. GFP reporter gene was inserted into the downstream of recombinant expression plasmid of P gene, which of stop codon was deleted. The experiment of transfection of P and GFP recombinant plasmid on COS-1 cells and CEF showed that GFP gene expressed, and this demonstrated that P gene was also expressed. This research may be helpful for further study of reverse genetics and functional genome of NDV of goose origin.
