THE IMPACT OF FRAMESHIFT MUTAIONS WITHIN mhbR AND mhbT ON THE CATABOLISM OF 3-HYDROXYBENZOATE
- VernacularTitle:mhbR和mhbT基因移码突变对3-羟基苯甲酸代谢的影响
- Author:
Xiao-Li GAO
;
Hong LIU
;
Shu-Jun WANG
;
Ning-Yi ZHOU
;
- Publication Type:Journal Article
- Keywords:
Hydroxybenzoate catabolism, Gentisate pathway, Frameshift mutation
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Klebsiella pneumoniae M5a1 metabolizes 3-hydroxybenzoate via gentisate to central metabolites. The plasmid pBSI encoding the full catabolic pathway endowed E.coli with the ability to grow on 3-hydroxybenzoate. We have determined the sequence of an 8-kb region spanning the mhb genes. Blast search in the GenBank revealed the mhbR was a putative transcript regulator and mhbT was a putative 3-hydroxybenzoate transporter. The E.coli strain containing the plasmid with frameshift mutantion either in mhbR or mhbT grew more slowly on 3-hydroxybenzoate than that containing the wild type clone.
