DETECTION OF FLAMMULIN WITH IMMUNOBLOT
- VernacularTitle:火菇素蛋白的免疫印迹检测
- Author:
Kai-Song ZHOU
;
Peng LV
;
Jiu-Gang XUE
;
Han-Xing ZHANG
;
Chang CHEN
;
Chang-Kai ZHANG
;
- Publication Type:Journal Article
- Keywords:
Flammulin, Flammulina velutipes, Immunoblot
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Flammulin, an anti-tumor protein, was purified from the aqueous extract of basidiomes of Flammulina Velutipes. Purified flammulin emulsified with Freund's adjuvant was injected subcutaneously into New Zealand white rabbits. After several immune enhancements, these animals were bled and sera were separated. Antiserum against flammulin in Western blots were applied to determine if flammulin be present in the liquid state culture or fruiting body. The result showed that anti-flammulin serum could recognize the aqueous extract of fruiting body in SDS-PAGE gels under the reducing conditions, no flammulin was detected in mycelia of Flammulina Velutipes.
