CLONING OF FULL CRY1C GENE FROM B.T.C.AND EXPRESSION IN CROPS BENEFICIAL BACTERIA BACILLUS CEREUS
- VernacularTitle:B.t.c. cry1C全长基因的克隆及其在增产菌中的表达
- Author:
Yue-Hua CHEN
;
Hong-Xiu LI
;
Jin-Hong WANG
;
Jun CAI
;
Gai-Xin REN
;
- Publication Type:Journal Article
- Keywords:
Bacillus thuringiensis subsp.colmeri, cry1C, Bacillus cereus, Spodoptera exigua
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Specific primers 1CaA/1CaB for full cry1C gene in Bacillus thuringiensis subsp.colmeri strain 15A3 were designed. The 4.0kb PCR product included the whole ORF and regulation region of cry1C gene. This PCR product was linked with shuttle vector pHT315 by two cloning steps. The recombined plasmid pHT-1C was electroporated into Bacillus cereus 9509, a kind of bacteria that beneficial to crops. The transformant could produce bipyramidal-shaped parasporal inclusions. The 60kD protein band was detected by SDS-PAGE. The bioassay result showed that the cry1C gene transformant of Bc 9509 had insecticidal activity to Spodoptera exigua.
