CLONING AND IDENTIFYING ANTIBIOTIC RESISTANCE RELATIONAL GENES OF NEISSERIA GENORRHOEAE
- VernacularTitle:耐药淋球菌相关核苷酸序列的克隆与初步分析
- Author:
Ming-Chun JI
;
Li QIAN
;
Hong-Ju CHEN
;
- Publication Type:Journal Article
- Keywords:
Neisseria gonorrhoeae,Antibiotic, Resistence,Genes
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To clone the differential genes in antibiotic resistence Neisseria gonorrhoeae,the library that contains the fragments of differential genes between antibiotic resistance strain and standard reference strain of Neisseria gonorrhoeae was constructed which used suppression subtractive hybridization technique. Then the antibiotic resistance relational genes fragments were cloned and analyzed. Antibiotic resistance Neisseria gonorrhoeae subtractive library that has high subtractive efficiency was set up successfully. The amplified library contained 2500 positive clones. Sequence analysis was performed to find the fragments of antibiotic resistance relational genes. Five sequences were unknown previously. The fragments of antibiotic resistance genes may provide an important clue for studying the mechanism of occurrence and development of antibiotic resistance Neisseria gonorrhoeae.
