CLONING AND SEQUENCING ANALYSIS OF GINGIPAIN K OF PORPHYROMONAS GINGIVALIS
- VernacularTitle:牙龈卟啉菌牙龈蛋白酶K的基因克隆和序列分析
- Author:
Feng-Qiu ZHANG
;
Lian-Jia YANG
;
Zhi-Fen WU
;
Ju-Cai YANG
;
- Publication Type:Journal Article
- Keywords:
Porphyromonas gingivalis,Gingipain K,Sequencing and Characterization
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The desired DNA product of KGPcd and KGP-hag was obtained from the total DNA of Porphyromonas gingivalis by PCR with two pairs of gene specific primers. The segment of KGPcd and KGP-hag (about 1.5kb and 1.6kb) was inserted into pGEM-T easy Vector. The double-stranded DNA of the postitive clone was analyzed by restriction endonuclease mapping and DNA sequenceing. The sequences of KGPcd and KGP-hag were consistent with those of the references appeared. The proteins of KGPcd and KGP-hag will be obtained for further study.
