A SIMPLE METHOD OF TRANSCONJUGATIVE CLONING WITH HIGH EFFICIENCY
- VernacularTitle:一种简单高效的结合转移克隆方法
- Author:
Zhao-Xing SHI
;
Heng-Liang WANG
;
Kun HU
;
Er-Ling FENG
;
Xiao YAO
;
Guo-Fu SU
;
Liu-Yu HUANG
;
- Publication Type:Journal Article
- Keywords:
Transconjugation, Cloning, Retromobilization
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to establish a method by which the recombinant suicide plasmids integrated on the chromosome could be recircled, A simple method of transconjugative cloning was established with the helper plasmids pMT999 or pRK2013 and fusion strains of Shigella flexneri which were obtained by screening with in vivo expression technology. And the cloning efficiency with this method is very high.
