Optimization of conus genomic DNA isolation methods
- VernacularTitle:芋螺基因组DNA提取方法的优化
- Author:
Sulan LUO
;
Ben ZHANG
;
Dongting ZHANGSUN
;
- Publication Type:Journal Article
- Keywords:
Conus;
genomic DNA isolation;
optimization
- From:
Chinese Journal of Marine Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Conopeptides (Conotoxin) derived from tropical marine gastropod, cone snail ( Conus ) venoms have been an useful tool agent in neuro investigation and new drug source. Recently, new method of Conotoxin genes cloning from Conus genomic DNA has been established. So to get Conus genomic DNAs rapidly is the basis for diverse Conotoxin gene isolation, gene bank construction and the medical source exploitation. In this report, different DNA extractions from different tissue and organs of six cone snail species were performed in order to optimize the DNA isolation methods. The optimized brief phenol SDS method was established which fit for cone snails genomic DNA extraction.
