EXTRACTION OF DNA FROM SOIL FOR PCR AMPLIFICATION
- VernacularTitle:从土壤中提取DNA用于PCR扩增
- Author:
Tao DU
;
Xiao-Mao HUANG
;
Ming-Sheng HOU
;
Mu-Lan LIN
;
Ning-Yi ZHOU
;
- Publication Type:Journal Article
- Keywords:
Soil, DNA, Extaction, PCR
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, five different methods were carried out for DNA extraction directly from soil. The result shows that all five methods could generate DNA with more than 15 kb in size. They were subsequently used as templates for PCR amplification with success, using primers of the bacterial 16S rRNA gene and Shiva-1 gene encoding an antibacterial peptide. However, method 5 is more suitable for DNA extraction directly from a small amount of soil sample as it produced a good yield of DNA in high integrity with reliable reproducibility.
