EVALUATION OF THE FEASIBILITY OF USING LACTOSE AS THE INDUCER IN PRODUCING THE VIRAL ENHANCING FACTOR FROM HELICOVERPA ARMIGERA GRANULOSIS VIRUS IN ESCHERICHIA COLI BL21DE3
- VernacularTitle:乳糖作为棉铃虫颗粒体病毒增效蛋白表达诱导剂的可行性研究
- Author:
Lin-Qian DONG
;
Ke-Qin ZHANG
;
Bing-Sheng QIU
;
- Publication Type:Journal Article
- Keywords:
Lactose, IPTG, ENHANCIN, T7 promoter, lacUVS promoter
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Lactose was shown to no less competent than Isopropyl-?-D-thiogalactoside (IPTG) in inducing the expression of the ENHANCIN coding gene from Helicoverpa armigera granulosis virus in Eswcherichia coli BL21 (DE3) regulated by a T7 promoter, since the lactose induction could lead to an ENHANCIN band no smaller than the one in IPTG induction on the SDS-PAGE gel. This would decrease the cost of the large-scale ENHANCIN production. The lactose concentration was optimized at 2.2% - 2.5% (w/v) . Different treatments on the lactose sterilization showed that lactose steam- sterilized in 116. 5℃ for 15min could lead to the ENHANCIN production. The convenience and the relatively low cost in its" operation could further decrease the cost of the ENHANCIN production.
