BIOSYNTHESIS OF EMULSIEIER BY A NEWLY ISOLATED RHODOCOCCUS RUBER STRAIN Eml
- VernacularTitle:生物乳化剂产生菌及其产乳化剂条件初步研究
- Author:
Xi-Wu LI
;
Zhi-Pei LIU
;
Shuang-Jiang LIU
;
- Publication Type:Journal Article
- Keywords:
Rhodococcus ruber, Bioemulsifler, Petroleum contamination, Degradation of hydrocarbons
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
A bacterial strain Eml isolated from contaminated soil of Shengli oil field was identified as Rhodococcus ruber according to its phenotype, physiological and chemical properties, and its 16S rRNA gene sequence. This strain could degrade various polycyclic aromatic hydrocarbons as well as alkanes in petroleum, and produce bioemulsifier. The results indicated that strain Eml could produce bioemulsifier efficiently when n-hexadecane was used as sole carbon source. The optimal conditions for the synthesis of bioemulsifier were as followed: 10g/L of n-hexadecane, 1g/L of yeast extract, media initial pH 7, and cultivation was carried out at 30℃ on a rotary shaker at 200 rpm. Under these conditions the surface tension of culture decreased to the lowest value, around 30 mN/m, after 1 day, and the emulsifying capacity was 100% . The concentration of bioemulsifier reached to the highest value, around 68 times of CMC , after 5 days' cultivation. The results also showed that the bioemulsifier produced by this strain should be lipid.
