THE EFFECT OF IMMOBILIZATION PROCESS TO THE ACTIVITY OF MTHASE AND MTSASE
- VernacularTitle:海藻糖合成酶活性受固定化处理过程影响的研究
- Author:
Jin-Hu FENG
;
Qi-Peng YUAN
;
Yan ZHOU
;
Zhong-Ming QIAN
;
- Publication Type:Journal Article
- Keywords:
Trehalose, Immobilization, MTSase, MTHase, Enzyme activity
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Amylum can be transformed into trehalose on the action of the MTSase and MTHase, however, the percentage of transformation is low. The research on the enzyme immobilization through four kinds of immobilization carrier is described in this paper. The result shows that the carriers, which are put into with enzyme liquid and crossbonded with chi-tosan and glutaraldehyde at first, can adsorb many enzymes independent from this process of trehalose synthesizing, consequently, the enzyme activity is increased sharply. By comparing with the influence of the immobilizing process and some factors in the reaction conditions, such optimize condition can be reached: the time for carrier crossbonding with glutaraldehyde is 18hours , the time with enzyme is another 18hours , and the time of reaction with amylum (10%) is 9hours.The result of the content of trehalose can reach 27.22g/L and the percentage of transformation can reach 54.43% , which previously is 2.67g/L and 5.33% respectively before dealt with by carriers.
