APPLICATION OF FLUORESCENCE IN SITU HYBRIDIZATION (FISH) IN MICROBIAL ECOLOGY
- VernacularTitle:FISH技术在微生物生态学中的研究及进展
- Author:
De-Feng XING
;
Nan-Qi REN
;
Ai-Jie WANG
;
- Publication Type:Journal Article
- Keywords:
Fluorescence in situ hybridization (FISH), 16S rRNA, Probe, PNA, Microbial molecular ecology
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Molecular technologies are sensitive, fast, and cheap in the study on microbial ecology. However, these methods do not provide information about morphology, number, and spatial distribution of the microorganisms. In contrast, Fluorescence in situ hybridization (FISH) combines the precision of molecular biology with the visual information from microscope, and permits visualization and identification of individual miciobial cells within their natural habitat. FISH not only allows the detection of slow growing microorganisms, but also of yet-to-be cultured. It is useful for many applications in diagnosis and assessment of the population structure of complex microbial communities, and is a powerful tool for molecular ecology studies in microbiology, In this paper, major techniques and progresses of FTSH were described. Its application in microbial ecology, as well as problems , pitfalls, and perspectives of FISH are discussed.
