Down-regulation of telomerase activity reflects neoadjuvant chemotherapy effect in invasive ductal carcinoma of breast
- VernacularTitle:新辅助化疗对乳腺浸润性导管癌端粒酶活性的影响
- Author:
Zeping YU
;
Yousheng LI
;
Chunhua GUO
;
Jieshou LI
;
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Telomerase;
Neoadjuvant chemotherapy
- From:
Journal of Medical Postgraduates
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the significance of telomerase activity in infiltrating ductal carcinoma with respect to neoadjuvant chemotherapy effect. Methods:Fine needle aspiration (FNA) specimens were obtained from twenty nine patients with primary breast cancer pre neoadjuvant chemotherapy.All patients were given neoadjuvant chemotherapy(CEF regimen, Cyclophosphamide 500 mg/m 2 ; Epirubicin 50～60 mg/m 2 , Fluorouracil 500 mg/m 2 ) and were performed operation on post chemotherapy 3th day to 5th day,cytology and telomerase activity of specimens were analyzed. The telomerase activity was measured Telomerase PCR ELISA based on a polymerase chain reaction (PCR) assay known as TRAP (telomeric repeat and amplification protocol) assay. Results:The optic density (OD) of telomerase activityis is 1.172?0.501 pre chemotherapy whereas post chemotherapy that is 0.771 ?0.442 ( P
