Detection of HLA-DRB 1*12 by use of SYBR Green I PCR
- VernacularTitle:SYBR GreenⅠ聚合酶链反应检测人白细胞抗原-DRB1*12
- Author:
Junyan LI
;
Jinming LI
;
- Publication Type:Journal Article
- Keywords:
Human leukocyte antigens;
Genotyping;
Polymerase chain reaction;
Melting curve
- From:
Chinese Journal of Laboratory Medicine
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the HLA DRB1*12 by use of a new genotyping method for HLA SYBR Green Ⅰ PCR. Methods 1 HLA DRB1*12 sample and 12 unknown clinical sample were collected. The typing of these samples were determined by PCR, which includes a fluorescence dye, SYBR GreenⅠ,and the sequence specific primer. SYBR GreenⅠcould bind to the dsDNA to exhibit fluorescence. The fluorescence enhancement depends on the accumulation of the amplified product. Results 3 of the 12 unknown sample was proved to be DRB1*12 by the analysis of the melting curve of the amplified products. After purification, the type of these products were confirmed DRB1*12 by DNA based sequencing. Conclusions The results of SYBR GreenⅠPCR demonstrate that it can be a new strategy for HLA typing because of its convenience and accuracy.
