EXTRACTION AND STRUCTURAL STUDY OF SCHIZOPHYLLAN FROM SCHIZOPHYLLUM COMMUNE BY SUBMERGED CULTIVATION
- VernacularTitle:深层培养裂褶菌胞外多糖的提取及结构研究
- Author:
Yi-Zhi JI
;
Lian-Xiang DU
;
- Publication Type:Journal Article
- Keywords:
Schizophyllum commune, Schizophyllan, Extract ion, Structure
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The extraction method and structure of exo-polysoccharide from schizophyllum commune by submerged cultivation were studied. Proteins were removed com p letely from the polysoccharide by the sevag method following the isoelectric poi nt precipitation method. The purified schizophyllan was proved to be homogeneou s with molecular weight 4?10 4D by sephadex G-200 column chromatography, PAGE and HPLC. Its monomer was determined by hydrolysis, PC, GC and its structure wa s analyzed by IR, enzymolysis, periodate oxidation, the results showed that schi zophyllan was only composed of glucose and it was the ?-glucan consisting of ?-(1-3) and ?-(1-6) glucosidic linkages.
