IDENTIFICATION OF ENDOPHYTES FROM TOMATO SEEDLINGS AND ANALYSIS OF DNA FINGERPRINTS USING ERIC-PCR
- VernacularTitle:番茄内生菌分离及其ERIC-PCR指纹图谱分析
- Author:
Yan-Qin LI
;
Quan SHEN
;
Bin-Bin LIU
;
Hua ZHANG
;
Li-Ping ZHAO
;
- Publication Type:Journal Article
- Keywords:
Tomato endophyte,Identify and purified,ERIC -PCR
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
A new method of identification and study of diversity of endophytes wa s described in this paper. The seedlings of tomato were treated using four meth o ds, sterile water, chemical reagents, ultraviolet radiation and mechanical to pe el off, respectively. Then endophytes were isolated using plates of beef- pept on e, Gaos I and PDA respectively. The optimal method of wiping off non-endophyte s was determined. We had obtained 148 isolates of endophytes with different size, shape and color. 43 strains of 148 were amplified using method of ERIC-PCR. T he results showed that 32 strains with amplified bands could fall into 28 classes. The purified bacteria were cultured confronting to leaf blight pathogen of tomat o to screen high resistant strain. Three bacteria strains with high resistance to pathogen were obtained.
