A MICROWAVE-BASED METHOD FOR GENOMIC DNA EXTRACTION FROM ACTINOMYCETES
- VernacularTitle:微波法快速提取放线菌基因组DNA
- Author:
Ping XU
;
Wenjun LI
;
Lihua XU
;
Chenglin JIANG
;
- Publication Type:Journal Article
- Keywords:
Microwave, Genomic DNA extraction, PCR, 16S rDNA
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The original methods on DNA extraction are not only complex and expens ive, but although invalid with actinomycetes under extreme environments Genomi c DNA can be extracted directly from biomass picked up from solid medium by micro wave thermal shock The extracted DNA is suitable for PCR The method is effec ti ve, easy and fast, and can be used in actinomycete systematics and identificatio n rapidly
