Molecular cloning and sequencing of apoA1 gene
- VernacularTitle:载脂蛋白A1基因克隆及序列分析
- Author:
Rong FANG
;
Baikun MA
;
Yufeng HUANG
;
- Publication Type:Journal Article
- Keywords:
apoA1;
Gene clone;
RT PCR
- From:
Journal of Medical Postgraduates
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:Identification, clone and sequence of the chinese mature apoA1 peptide gene.Methods:Total RNA was prepared from Chinese fetal liver tissue, cDNA fragment encoding human apoA1 was amplified by RT PCR using specific primers, and cloned into pGEM T vector. Inserted apoA1 gene was sequenced by ABI 377 DNA Sequencer. Results:Analysis indicates that the DNA fragment is 739 base pairs in length and has 100% nucleotide homology with that reported previously. Conclusions:Human apoA1 gene was successfully cloned from fetal liver tissue.
