Construction and identification of rGA antisense eukaryotic expression vector
- VernacularTitle:大鼠谷氨酰胺酶反义真核表达载体的构建及鉴定
- Author:
Hongming LIU
;
Juntao YANG
;
Hongguang GU
;
- Publication Type:Journal Article
- Keywords:
Glutaminase;
Antisense eukaryotic expression vector;
Rat
- From:
Journal of Medical Postgraduates
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To construct rGA antisense eukaryotic expression vector. Methods:Using molecular cloning technology, we subcloned rGA cDNA in its antisense orientations of the pcDNA 3.0 vector. Results:Cutting with Eco RⅠ and Hin D Ⅲ,antisense recombinant plasmid produce 2 bands. One was 0.28 kb fragment, the other was 5.5 kb fragment which corresponded to theoretical calculation. Conclusions:We succeeded in constructing rGA antisense eukaryotic expression vector.
