STUDY ON RESISTANCE GENE KNOCK OUT FROM INTEGRATED ALKALINE PROTEASE GENE ENGINEERING STRAIN
- VernacularTitle:整合型碱性蛋白酶基因工程菌中抗性基因的敲除
- Author:
Xueming TANG
;
Weilan SHAO
;
Zhengxiang WANG
;
Huiying FANG
;
Jian ZHUGE
;
- Publication Type:Journal Article
- Keywords:
Alkaline protease, Gene engineering strain BP071, Resistance gene, Knock out
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The knock out vector pHK was constructed with E coli vector pET 28a and shuttle vector pHY300PL, by using denatured DNA and homologous recombination technique, the kanamycin resistance gene ( Kan r) from integrated alkaline protease gene engineering strain BP071 was knocked out successfully, and the 11 positive clones were obtained The yield of the best positive clone BP0715 was stable as same as BP071 The methods provided the good experience for the industrial microbiology research, and it was foundation for studying on the safety of genetically modified organisms
