RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) OF ADHESIN GENE HPA A FROM DIFFERENT HELICOBACTER PYLORI STRAINS
- VernacularTitle:幽门螺杆菌hpaA基因RFLP研究
- Author:
Yu HONG
;
Quanming ZOU
;
Xuhu MAO
;
Shenrong JING
;
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori , Adhesin, hpaA , RFLP
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To assess the variability of adhesin gene hpaA in differene H pyloristrains with PCR restriction fragment length polymorphism (RFLP) A 710 bp gene hpaA , obtained from 9 different H pylori strains, were digested by Hha Ⅰand Hae Ⅲ individually and analyzed by agarose gel electrophoresis Four different polymorphic types were found in hpaA digested with Hae III and five types with Hha I Clinical isolates of H pylori from Chongqing showed difference among them and remarkably distinguished from foreign standard strains Mongolia gerbil adapted H pylori strain,which were obtained from Mongolia gerbil infected with clinical isolate, also showed inconsistence in hpaA RFLP The hpaA gene from different H pylori strains revealed 1 variability, and this might provide an effective method for developing molecular epidemiology of H pylori
