THE REMOVAL OF LARGE INVASIVE PLASMIDS FROM SHIGELLA SPECIES
- VernacularTitle:驱除痢疾杆菌侵袭大质粒的新方法
- Author:
Erling FENG
;
Xiang LIAO
;
Hengliang WANG
;
Zhaoxing SHI
;
Guo SU
;
Liuyu HUANG
;
- Publication Type:Journal Article
- Keywords:
Shigella species , Invasive plasmid, Incompatibility
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to remove invasive plasmids from Shigella flexneri 2a 2457T and Shigella sonnei S7 based on the principle of plasmid incompatibility Ori and inc genes were amplified by PCR from S. flexneri 2a invasive plasmids, and then they were cloned into plasmid pMD18 T, resulting recombinant plasmids pMDori and pMDinc After S flexneri 2a 2457T and S sonnei S7 were transformed with pMDori or pMDinc respectively Invasive plasmids were removed from S flexneri 2a 2457T and S sonnei S7
