Effects of post-burn plasma on signal molecule, NF-?B activation and interleukin 6 expression in cultured PBMC of rats
- VernacularTitle:钙拮抗药及烧伤血浆对大鼠PBMC内NF-?B活化和炎症介质产生的影响
- Author:
Baojun YU
;
Zhaofan XIA
;
Jieshou LI
;
- Publication Type:Journal Article
- Keywords:
Burn;
PBMC;
Calcium ion;
Nuclear factor ?B;
Nimodipine;
Dantrium ○R
- From:
Journal of Medical Postgraduates
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To study the alterations of calcium ion and NF ?B activation in cultured PBMC after stimulation of post burn plasma, and to investigate the roles of signal transduction pathway in the mechanisms of SIRS after burn. Methods:PBMC were stimulated with post burn plasma in vitro . The calcium ion concentration were measured by fura 2. The NF ?B protein were measured by EMSA. Results:Lots of NF ?B proteins were sequester in nucleus in all burn plasma groups. Dantrium and Nimodipine could decrease the activity of NF ?B protein in nucleus, and have significant inhibitory effects on the production of interleukin 6 and NO. Conclusions:In signal transduction levels, calcium ion may modulate the activity of NF ?B which mediate the expression of most proinflammtory cytokines.
