The growth-suppressive effect of retrovirus-mediated wild-type p53 gene on human esophageal cancer cell line
- VernacularTitle:逆转录病毒介导的p53基因对食管癌细胞株的生长抑制作用
- Author:
Yagang ZHAO
;
Guihua SUN
;
Hongbin ZHANG
;
Meihua ZHOU
;
Lan ZHOU
;
- Publication Type:Journal Article
- Keywords:
Genes p53;
Gene therapy;
Esophageal neoplasms;
Retrovirus
- From:
Journal of Medical Postgraduates
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To observe growth suppressive effects of human wild type p53(wt p53) gene on human esophageal cancer cell line. Methods:Using the retroviral vector to introduce exogenous wt p53 gene into human esophageal cancer cell line ECA109,the gene expression and tumor inhibition were studied in vitro and in vivo . Results:The expression of p53 in transfected cell lines(ECA 109/p53 ) was increased.The growth rates and the ability to form colony in soft agar were greatly inhibited in ECA 109/p53 cells versus ECA109/neo and ECA109 cells.The G 0+G 1 ratio increased and S ratio decreased in cell cycle distribution,and apoptosis index significantly rose in the ECA109/p53 cells,which were confirmed by FCM analyzing.The tumorigen icity of ECA109/p53 cells in nude mice was obviously suppressed by exogenous p53. Conclusions:Exogenous wt p53 mediated by retroviral vector could inhibit the growth of human esophageal cancer cell.
