STUDIES ON ISOLATION, PURIFICATION AND ANTITUMOR ACTIVITY OF POLYSACCHARIDES OF PLEUROTUS FERULAE
- VernacularTitle:阿魏侧耳多糖的分离纯化与抗肿瘤活性的研究
- Author:
Hongxin DONG
;
Zuozhou LU
;
- Publication Type:Journal Article
- Keywords:
Pleurotus ferulae , Polysaccharides, Isolation, Purification, Antitumor activity
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
PFW was isolated from fruit body of Pleurotus ferulae by using hot water Proteins were removed from PFW with Sevag method, after DEAE cellulose chromatography, PF 1 and PF 2 were isolated from PFW The weight was determined by using multiangle laser photometer combined with GPC; the results showed: PF 1 contains two fractions, the weights (Mw) are 7 875?10 5 and 3 245?10 4, the Mw of PF 2 is 3 187?10 6 The components and structure of polysaccharides were analyzed by IR and GC The polysaccharides PF 1 consists of rhamnose, glucose, galactose, mannose; PF 2 consists of rhamnose, xylose, glucose, galactose, mannose; PF 1 contains ? glucoside and mannoside, PF 2 contains ? glucoside It was found polysaccharides have antitumor activity on the implanted Sarcoma 180 in mice
