SYNTHESIS OF L-DOPA BY CITROBACTER FERUNDII RESTING CELLS
- VernacularTitle:Citrobacter freundii休止细胞催化合成L-多巴
- Author:
Li ZHANG
;
Wen LI
;
Zhenghua XIAO
;
Yunxia WANG
;
Shi XIA
;
- Publication Type:Journal Article
- Keywords:
L DOPA, Tyrosine phenol lyase, Microbial conversion
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The resting cells of Citrobacter freundii 48003 3 expressing high tyrosine phenol lyase activity under the inducing of L tyrosine were used for L DOPA synthesis from catechol, pyruvate and ammonia In this paper, the effects of temperature, pH and substrate concentrations on the synthesis of L DOPA were studied At the optimal conditions of reaction, 9 5g/L of L DOPA was obtained in 12h
