SCALE UP OF GUANOSINE FERMENTATION BASED ON SCALE-DOWN TECHNOLOGY
- VernacularTitle:应用规模缩小方法的鸟苷发酵过程放大
- Author:
Xianpeng CAI
;
Shuangxi CHEN
;
Ju CHU
;
Yingping ZHUANG
;
Si ZHANG
;
Yongmei LIU
;
Zhongshi WANG
;
- Publication Type:Journal Article
- Keywords:
Guanosine, Fermentation, Scale down, Scale up
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Based on the optimized technology on 50L scale, B subtilis 754# was used to manufacture guanosine on pilot scale (12M 3) and plant scale (100M 3) to give the production of 29 4g/L and 21 4g/L successively Through scale down the process of plant scale to pilot scale, based on the theory of dynamic metabolic flux, DO was found to be another key factor that limited the scale up process After conquering the DO limitation, the optimized technology was successfully scaled up to plant scale and the production was further increased by 18% to 25 2 g/L
