Effects of protein kinase-induced cytoplasmic calcium changes in mesangial cells on glomerular hy- perfiltration in early stage of rat diabetes mellitus
- VernacularTitle:蛋白激酶介导的系膜细胞内钙变化在早期糖尿病鼠肾小球高滤过中的作用
- Author:
Song-Min HUANG
;
Hai-Yan ZHANG
;
Yong XU
;
Zhao-Hui SHA
;
Li ZHOU
;
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Glomerular mesangial cells;
Calcium;
Glomerular hyper- filtration
- From:
Chinese Journal of Diabetes
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate functional alterations of glomerular mesangial cell (GMC) contraction and its relationship with diabetic glomerular hyperfiltration. Methods Male Wistar rats were divided into groups of normal control and 2 weeks diabetes induced by STZ. GMC were cultured in the presence of normal glucose (5mM). Meanwhile in comparison GMC obstained from normal rats were cultured in the presence of 30 mM glucose for 48 h in vitro. By using laser scanning confocal microscopy (LSCM) , we measured basal calcium concentration, the changes of calcium level in response to vasoconstrictor agonist angiotensin H (Ang I ),and the effect of protein kinase C (PKC) inhibitor PMAC10 M,24 h)on cytosol calcium of GMCs. Results The creatinine clearance rate (Ccr) was increased with age in diabetic rats, reaching a peak at 2 weeks (P
