Methylation State within MAGE-1 B′B Promoter in Gastric Carcinoma
- VernacularTitle:胃癌MAGE-1基因启动子B′B区去甲基化状态的研究
- Author:
Lin XU
;
Bing LUO
;
Qing WANG
;
Hefei XU
;
Weiqing HUANG
;
Hua LIANG
;
- Publication Type:Journal Article
- Keywords:
gastric carcinoma;
MAGE 1 gene promoter;
demethylation
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the methylation state within MAGE 1 B′B promoter in gastric carcinoma and the association between demethylation and pathological differentiation, the association between demethylation and clinical stage. Methods: Using methylation sensitive restriction analysis followed by polymerase chain reaction (PCR),we studied 80 specimen that were obtained from surgical samples (including 40 gastric carcinoma and 40 matched adjacent normal gastric mucosae).Results: An demethylation state was identified in DNA from gastric carcinoma specimens.The demethvlation rate is 25%(10/40).In contrast,no demethylation state was identified in DNA from matched adjacent normal gastric mucosae. The differences were Significant statistically. ln our study, the demethylation in poorly, moderately, and well differentiated glandulous cell carcinoma were detected at frequencies of 50%,18.7% and 8.3%,respectively, The differences were significant statistically ( P
