All Trans Retinoid Acid Inhibit Cell Growth in Human Retinoblastoma Cells Via Phosphorylation of JNK
- VernacularTitle:全反式维甲酸通过磷酸化JNK抑制视网膜母细胞瘤细胞生长
- Author:
Hongbo MIN
;
Jianwen WANG
;
Jihu SUN
;
Shizhong BU
;
Yongwei YU
;
- Publication Type:Journal Article
- Keywords:
all trans retinoid acid;
JNK;
growth inhibition;
Y79
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate all trans retinoid Acid (ATRA) inhibition of cell growth in human retinoblastoma Y79 cells, and its mechanism. Methods: Antiproliferation effects of ATRA on Y79 cells were determined by 3 H thymidine incorporation. Cell cycle analysis was performed by flow cytometry. JNK phosphorylation was analyzed by Western blot analysis. Results: After 36 h treatment with 10 -6 mol?L -1 ATRA, 3 H thymidine incorporation decreased to 40%, under the same condition, Y79 cells were arrested in G 0 /G 1 and Sub G 1 peak appeared. Curcumin, JNK blocker, blocked the growth inhibition by ATRA. JNK was phosphorylated in 10 to 20 min. Conclusion: JNK phosphorylating mediated ATRA inhibition of apoptosis in Y79 cells. These results suggested that ATRA might have clinical application for treatment of retinoblastoma.
