THE ISOLATION AND RESEARCH OF NITROBENZENE DEGRADATIVEPLASMID FROM PSEUDOMONAS XN-1
- VernacularTitle:假单胞菌XN-1硝基苯降解性质粒的提取及研究
- Author:
Chun LIU
;
Jiti ZHOU
;
Liping HUANG
;
Hong LV
;
- Publication Type:Journal Article
- Keywords:
Pseudomonas XN 1, Plasmids,Mutants,Nitrobenzene degradation
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Pseudomonas XN 1 has the ability of degradation for organic pollutant nitrobenzene, and the resistance to ampicillin In this paper, the plasmid was detected and isolated from the cell of Pseudomonas XN 1, and plasmid pXN 1 whose size is about 22 kb was gained The experiment of plasmid elimination confirmed that the plasmid has some relation to the ability of nitrobenzene degradation, while it is irrelevant to the resistance to ampicillin The feature difference between XN 1and its spontaneous mutants was corresponding to the difference of the plasmids detection, and a derivative plasmid pXN 13 was obtained from mutant XN 13 which is a few kb smaller than pXN 1
