SEQUENCS ANALYSIS OF RIBOSOMAL PROTEIN GENE OF PHYTOPLASMA ASSOCIATED WITH PERIWINCLE YELLOWS
- VernacularTitle:长春花黄化植原体核糖体蛋白基因片段序列分析
- Author:
Hong CAI
;
Hui CHEN
;
Fan LI
;
Hairu CHEN
;
- Publication Type:Journal Article
- Keywords:
Periwinkle, Yellows, Phytoplasma, Ribosomal protein gene, Sequence analysis
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A 1 3kb DNA fragment was amplified in DNA samples extracted from periwinkle showed yellows The amplified fragment was ligated into pGEM T easy vector and then transformed into JM109 strain of E coli Cloned DNA fragments were verified by PCR, restriction endonuclease ( Eco RI) digestion and sequence analysis The result revealed that the ribosomal protein gene of PY strain consists of 1244 base pairs, which contains the entire rp l22 gene encoding 129 amino acids and rps3 gene encoding 252 amino acids These two genes are overlap The character of ribosomal protein gene of PY strain is very resemble with that of other phytoplasmas
