SCREENING OF MUTANT OVERPRODUCING GLA WITH RESISTANCE METHODSCREENING OF MUTANT OVERPRODUCING GLA WITH RESISTANCE METHOD[WT9
- VernacularTitle:用抗性筛选法选育?-亚麻酸(GLA)高产菌株
- Author:
Bo CHEN
;
Ling ZHANG
;
Xinsheng HE
;
Daifa LI
;
Xi WANG
;
- Publication Type:Journal Article
- Keywords:
Mortierella isabellina ,UV mutagenesis,Resistance screen,Mutant
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Using Mortierella isabellina as starting strain, mutagenized with UV,screened by selecting mutants resisting maleic hydrazide,a fatty acid dehydrogenase inhibitor, directly on gradient plate,a mutant M 80 overproducing Gamma Linolenic Acid (GLA) was obtained, which has much higher productive qualities than its parental strain Its dry cell weight reaches 25 10g/L,lipid yield 12 35g/L,and GLA yield 771 88mg/L
