Molecular biological on rifampim-depending M.Tuberculosis straims isolated from patients
- VernacularTitle:依赖利福平结核分枝杆菌的分子生物学研究
- Author:
Min ZHONG
;
Bo-Hai WEN
;
Rong CHEN
;
Wei CHEN
;
Yiwei WANG
;
An-Rong WANG
;
Ming ZHONG
;
- Publication Type:Journal Article
- Keywords:
Mycobacterium,tuberculosis;
Rifampin;
Gene;
DNA Fingerprinting;
Electrophoresis, polyadrylamide gel
- From:
Chinese Journal of Laboratory Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular biology of rifampin-depending M. Tuberculosis. Methods The seguence (a 319-bp DNA fragment) of rpoB gene were analyzed by automated DNA sequencing machine. (2) The fingerprints of genomic DNA were obtained by random amplified polymorphic DNA (RAPD) fingerprinting. (3)The protein electrophoresis of bacterium by SDS-polyacrylamide gel (SDS-PAG).(4) The cases of pulmonary tuberculosis by rifampin-depending strains were retrospectively analyzed. Results (1) rpoB gene sequenced: The point mutationrate of rifampin-depending strainswas 96.7%(29/30) and that of rifampin-residtant strains 81.1%(30/37), P
