Immunoscreening and Sequence Analysis of a cDNA Library of Adult Trichinella spiralis
- VernacularTitle:旋毛虫成虫cDNA文库免疫筛选及序列分析
- Author:
Yaping YANG
;
Xinping ZHU
;
Jing YANG
;
Lei ZHOU
;
Song HUANG
;
- Publication Type:Journal Article
- Keywords:
Trichinella spiralis , adult worm, cDNA library, immunoscreening, sequence analysis
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain an antigenic gene of adult Trichinella spiralis. Methods cDNA library of the adult Trichinella spiralis was screened using the sera of immunized and infected rabbits. The gene sequence was analyzed by DNAstar software and GenBank database. Results Nine positive clones were identified by immunoscreening. The clone Ts87 was sequenced and a cDNA with 1 172 bp full length was obtained using 5′ RACE technique, encoding 347 amino acids. Some possible antigen epitopes were predicted. Conclusion A novel antigenic gene of Trichinella spiralis was obtained.
