The Effects of Calcitonin on Human Breast Cancer Cell Line T47D in vitro and in vivo
- VernacularTitle:降钙素对人乳腺癌细胞系T47D作用的体内外研究
- Author:
Li ZHAO
;
Yinglin CAO
;
Wensheng SUN
;
Zhiqiang ZHANG
;
- Publication Type:Journal Article
- Keywords:
calcitonin;
breast cancer;
apoptosis;
telomerase;
nude mice
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of calcitonin on human breast cancer cell line T47D. Methods: The inhibition rates of salmon calcitonin (sCT) on T47D cells were measured by MTT methods. Then telomerase activity of T47D cells was detected using PCR ELISA methods. Cell apoptosis was observed by transmission electron microscope (TEM). Animal models in vivo were constructed by implanting T47D cells subcutaneously into nude mice. After injection of sCT for 30 days, tumor diameters were measured. The structure of lumbar 3 were separated and compared by a scanning electron microscope (SEM). Results: The inhibitory effects of sCT on T47D cells was observed by MTT method. The PCR ELISA method discovered that sCT could decrease the telomerase activity of T47D cells. TEM found cell apoptosis induced by sCT. Tumor diameters in sCT treatment group showed no statistical difference compared with the control group. SEM of lumbar 3 discovered that sCT could strengthen the bone structure of nude mice. Conclusions: The decrease of telomerase activity and induction of apoptosis are new mechanisms of sCT inhibition on T47D cells. The tumor inhibition in vivo was not observed. This may be attributed to the complicated endocrine response in vivo . sCT is still effective in strengthening the bone structure of those nude mice without osteoporosis.
