STUDY ON THE CHARACTERS OF AN HARPIN-PRODUCING BACTERIA STRAIN TOLERANT TO AMMONIUM AND NITROGEN-FIXING
- VernacularTitle:产过敏素harpin的固氮工程菌的某些特性研究
- Author:
Hong-Hui ZHU
;
Yan-Qin LI
;
Xiao-Ying QIU
;
Ming-Qi QIU
;
Li-Ping ZHAO
;
- Publication Type:Journal Article
- Keywords:
Strain E4, HrpN gene, nifA gene, Ability of hypersensitivity-inducing, Stability of plasmid
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The ability to induce hypersensity on leaves of tomato and the stability of double-plasmid of an harpin-producing, nitrogen-fixing engineered strain E4 were tested. Hypelsensitivity-inducing experiment indicated that the time and density of hypersensitivity-induction of E4 was similar to those of DH5, the positive control of pCPP430. Although E4 took the same time to induce hypersensitivity as 308R, another positive control of pCPP430, it induced weaker hypersen- sitivity on tobacco leaves. On tomato leaves, there was no difference in time and density of hypersensitivity between E4 and 308R (pCPP430). Results revealed that the two plasmids, pCPP430 and pMC73A, were unstable in host bacteria, with the losing rate of 100% at the 48th generation. The emergence probability of bacteria with either pCPP430 or pMC73A was almost the same.
