STUDY ON THE FERMENTATION CONDITION PRODUCING 2-KETO-LGLUCONIC ACID BY USING MIXED CULTURE OF MICROORGANISM
- VernacularTitle:微生物混合培养从D-山梨醇产生维生素C前体—2-酮基-L-古龙酸发酵条件的研究
- Author:
Wen-Chu LIN
;
Qing YE
;
Chun-Hong QIAO
;
Guang-Lin YIN
;
- Publication Type:Journal Article
- Keywords:
Mixed culture, D-sorbitol, 2-keto-L-gluconic acid, Orthogonal experiments
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Vitamin C precusor-2-keto-L-gulonic acid can be prepared directly by mixed culture of Ghiconobacter oxy-dans SCB329 and Guconobacter subaxydans SCB110. To obtained its high yield, firstly, the proportion of the two micro- organisms, the ingredients of medium and the initial pH were optimized in shake flaskd, then L9 (34) orthogonal experiment confirmed that urea, C. S. L had high degree statistical meaning. Based on these data, an optimized fermentation media was obta ined: D-Sorbitol 9g, C. S.L1.5g, Urea1.5g, KH2PO40.1g, CaCO30.2g. By-product can be inhabited to the greatest extent and the yield increases by 20%.
