STUDIES ON THE EXPRESSION CONDITION OF SEGMENT OF GABA_A RECEPTOR a1 SUBUNTT IN E. COLI
- VernacularTitle:GABA_A受体蛋白片段在大肠杆菌细胞表达条件研究
- Author:
Ya-Zhong XIAO
;
Jun HANG
;
Hong XUE
;
- Publication Type:Journal Article
- Keywords:
GABAA Receptor protein, E. coli, Expression condition
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to improve the expression level of segment of GABAA receptor a1 subunit in E. coli, growth conditions of the recombatant, which influence the final yield of protein expression, including growth medium, inoculation ratio, temperature, pH, rotation speed, inducing time and concentration of IPTG and so on, were studied in shaking flasks. The results indicated that, with 3% inoculation ratio, cultured 3.5 hours at 37℃, and then induced 5 hours by IPTG at 32℃, the yield of GABAA receptor protein was 95mg/L and the biomass was 3.25 g/ L. In contrast, using a 16 L stirred fermentor instead of shaking flasks, the highest level of the protein expression, 136mg/L with 4.95g/L of biomass, was achieved after fermenting 5.5 hours.
