COMPARATIVE ANALYSIS OF NUMERICAL TAXONOMY AND 16S rDNA PCR-RFLP OF FAST-GROWING RHIZOBIA ISOLATED FROM WESTERN CHINA
- VernacularTitle:西部某些根瘤菌的数值分类和16SrDNA PCR-RFLP分析
- Author:
Feng-Ling KAN
;
Wen-Xin CHEN
;
- Publication Type:Journal Article
- Keywords:
Rhizobia, Numerical Taxonomy, 16S rDNA PCR-RFLP
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
strains of fast-growing rhizobia isolated from Chinas western (mainly from Qinghai province),and 4 representative strains were compared by performing numerical taxonomy. 132 phenotypic characteristics were analyzed. The results of numerical taxonomy constructed a dendrogram. It shows that all the strains examined clustered into five phena at a similarity level of 79%. 16S rDNA PCR-RFLP were applied to 57 rhizobial strains (among the 61 strains) and 10 reference strains. The 16S rDNA-RFLP analysis described 20 different genotypic characteristics and form one dendrogram. For some large groups, the results of 16S rDNA-RFLP were agreement with that of numerical analysis of phenotypic characteristics.
